hGIIA and HSPGs localize to soluble fractions of sequentially lysed apoptotic T cells. hGIIA was incubated with control or anti-Fas–treated human T cells for 15 minutes and washed. Cells were then lysed sequentially with hypotonic and hypertonic lysis buffers. Soluble fractions and the pellet from cells treated with hypertonic lysis buffer were prepared as described in “Materials and methods.” Proteins in the samples were separated by SDS-PAGE and transferred to PVDF membranes. The membranes were probed with anti-hGIIA (A) or anti-HSPG antibodies (B) as described in “Materials and methods.”