hGIIA binding to purified vimentin is not inhibited by heparin. Indicated amounts of vimentin were spotted on a PVDF membrane and incubated with hGIIA (0.1 μg/mL) in the absence (top panel) or the presence (bottom panel) of heparin (50 μg/mL). The membrane was washed and probed with an anti-hGIIA antibody as described in “Materials and methods.”