Characterization of irradiated LDLR KO mice transferred with either CD2-dnTGFBRII or control bone marrow. (A) PCR was performed using primers indicated in Figure 1 on DNA isolated from splenocytes of LDLR KO mice transferred with either a control bone marrow (1-4) or a CD2-dnTGFBRII bone marrow (5-8) to show the effective transfer and reconstitution of mice with the bone marrow of CD2-dnTGFBRII transgenic mice. Line 9 indicates no DNA; line 10, no PCR; line 11, molecular weight. (B) Proliferative response and TGF-β function of splenocytes from control mice or mice reconstituted with CD2-dnTGFBRII transgenic bone marrow. Purified T cells isolated from spleens of control mice (▪) or CD2-dnTGFBRII reconstituted mice (▨) were stimulated with both con A (0.5 μg/mL) and irradiated T-depleted syngeneic normal splenocytes in the presence or absence of TGF-β1 (1 ng/mL) as indicated. Proliferation of triplicate culture was measured at day 3. P = .004 between control and dnTGFBR in the absence of TGF-β1; P = .0002 between control with and control without TGF-β1. Numbers above bars indicate mean values. Error bars show SEM.