Caspases 8 and 10 are not activated by LPS. (A) DCs were exposed to LPS (100 ng/mL) or staurosporine (10 μM), with or without pretreatment with zVAD (1 hour, 40 μM), and activation of caspases 8 and 10 was analyzed by Western blotting at the indicated time points. Three independent experiments gave similar results. (B) DCs were exposed to LPS (100 ng/mL) or staurosporine (1 μM) for the indicated time points. Cell lysates were then incubated with in vitro–translated [³⁵S]PARP, with or without 100 μM zVAD, and the products of the cleavage reaction were analyzed by autoradiography. Three independent experiments gave similar results. (C) DCs were left untreated or exposed to LPS (100 ng/mL) for 2 hours. Cell lysates were then incubated with in vitro–translated pro-IL1β, with or without 100 μM zVAD or YVAD, and the products of the cleavage reaction were analyzed by autoradiography. Two independent experiments gave similar results.