Figure 2.
Figure 2. The apoptotic hypersensitivity of Fancc-/- cells to TNF-α is p53 dependent. (A) Effect of p53 deficiency on the TNF-α-induced hypersensitivity of Fancc-/- progenitors. Hematopoietic progenitor colonies from bone marrow low density mononuclear cells were cultured in the presence of increasing amounts of TNF-α. Colonies were scored, and the data are plotted as percent of control CFU-GM colonies scored in the absence of TNF-α. Percent of control is shown on the y-axis, and the TNF-α dose is shown on the x-axis. The mean of each data point ± SD is shown. Each genotype is indicated by the corresponding symbol shown in the key. Statistics were assessed using the Student t test. The Fancc-/-Trp53+/+ genotypes were statistically different from all other groups (P < .001). A representative experiment conducted in triplicate cultures is shown. (B) Effect of p53 deficiency on the mitomycin C-induced hypersensitivity of Fancc-/- progenitors. Hematopoietic colonies were scored in the presence and absence of increasing doses of mitomycin C. The data are shown as percent of control (untreated) colony formation, and each genotype is indicated by the corresponding symbol shown in the legend. The mean of each data point ± SD is shown. The Fancc-/-Trp53-/- genotypes were statistically different from Fancc-/-Trp53+/+ progenitors from 2.5 to 50 nM mitomycin C concentrations (Student t test, P < .01). (C) The hypersensitivity of primary, Fancc-/- MEFs to TNF-α is dependent on p53. MEFs were cultured in the presence and absence of TNF-α (50 ng/mL), and were counted after 72 hours of culture. A representative experiment (n = 5) with similar results is shown. The change in cell number due to TNF-α treatment is expressed as percent of control, untreated cells for each genotype. Percent of control is shown on the y-axis, and the genotype is shown beneath each bar. Error bars indicate SD. Statistical analysis was performed using the Student t test. The differences between the Fancc+/+Trp53-/- and Fancc-/-Trp53-/- groups are not statistically significant.

The apoptotic hypersensitivity of Fancc-/- cells to TNF-α is p53 dependent. (A) Effect of p53 deficiency on the TNF-α-induced hypersensitivity of Fancc-/- progenitors. Hematopoietic progenitor colonies from bone marrow low density mononuclear cells were cultured in the presence of increasing amounts of TNF-α. Colonies were scored, and the data are plotted as percent of control CFU-GM colonies scored in the absence of TNF-α. Percent of control is shown on the y-axis, and the TNF-α dose is shown on the x-axis. The mean of each data point ± SD is shown. Each genotype is indicated by the corresponding symbol shown in the key. Statistics were assessed using the Student t test. The Fancc-/-Trp53+/+ genotypes were statistically different from all other groups (P < .001). A representative experiment conducted in triplicate cultures is shown. (B) Effect of p53 deficiency on the mitomycin C-induced hypersensitivity of Fancc-/- progenitors. Hematopoietic colonies were scored in the presence and absence of increasing doses of mitomycin C. The data are shown as percent of control (untreated) colony formation, and each genotype is indicated by the corresponding symbol shown in the legend. The mean of each data point ± SD is shown. The Fancc-/-Trp53-/- genotypes were statistically different from Fancc-/-Trp53+/+ progenitors from 2.5 to 50 nM mitomycin C concentrations (Student t test, P < .01). (C) The hypersensitivity of primary, Fancc-/- MEFs to TNF-α is dependent on p53. MEFs were cultured in the presence and absence of TNF-α (50 ng/mL), and were counted after 72 hours of culture. A representative experiment (n = 5) with similar results is shown. The change in cell number due to TNF-α treatment is expressed as percent of control, untreated cells for each genotype. Percent of control is shown on the y-axis, and the genotype is shown beneath each bar. Error bars indicate SD. Statistical analysis was performed using the Student t test. The differences between the Fancc+/+Trp53-/- and Fancc-/-Trp53-/- groups are not statistically significant.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal