Time courses of FXa, FVa, and thrombin generation in normal plasma. Plasma was triggered with approximately 6.4 ng/mL TF in the absence (⋄) and in the presence (♦) of 0.4 nM APC, and the generation of FXa (A), FVa (B), and thrombin (C) was followed in time with the subsampling method. Concentrations of activated coagulation factors refer to the reaction mixture.