Time courses of FXa, FVa, and thrombin generation in the plasmas of the FVII Lazio-homozygous patients. The plasmas of patient no. 3 (open symbols) and patient no. 6 (closed symbols) were triggered with approximately 6.4 ng/mL TF in the absence (A-C) and in the presence (D-F) of 0.4 nM APC, and the generation of FXa, FVa, and thrombin was followed in time by the subsampling method. Each curve represents the average of at least 2 measurements of the whole time course. Concentrations of activated coagulation factors refer to the reaction mixture.