FVII-titration of thrombin generation in plasma containing either normal FV or FV Leiden. FV-FVII doubly deficient plasma was supplemented with increasing amounts of FVII and reconstituted with either normal FV (open symbols and dashed lines) or FV Leiden (closed symbols and solid lines). Plasma was triggered with approximately 1.9 ng/mL TF in the presence of 10 nM APC and thrombin generation was followed continuously with a fluorogenic substrate. FVII concentrations were as follows: 0% (dashes), 0.1% (diamonds), 0.2% (squares), 0.5% (triangles), 1% (circles), and 10% (crosses/stars).