TCR BV repertoire in normal T cells and cell mixtures of these with Jurkat cells. (A) CDR3 spectratypes of T cells from a healthy donor showing highly diverse profiles reflecting a heterogeneous TCR repertoire. (B) Spectratype profiles of the cell mixtures containing 3% normal T cells and 97% Jurkat cells. As observed in panel A, polyclonal spectratype profiles were readily found across the other TCR BV subfamilies, even though 97% of the analyzed cells were composed of a clonal population (note the dominant peak in BV 8, representing Jurkat cells). (C) Spectratype profiles of BV 8 from pure normal T cells (1) and normal T cells spiked with increasing numbers of Jurkat cells (2-7). A single dominant peak was observed at a ratio of normal BV 8⁺ T cells to Jurkat cells of 1:333.