American Society of Hematology

Figure 1.

$Figure 1. RDR and GALV mRNA levels relative to β2-microglobulin mRNA in human apheresis product, cord blood, and bone marrow. (A) RDR mRNA levels were determined by RT TaqMan PCR in unfractionated (UF) mononuclear progenitor cells (mean, 0.1 ± 0.05), lin–CD34+CD38+ (mean, 0.18 ± 0.05), and lin–CD34+CD38– (mean, 0.20 ± 0.06) cells. (B) GALV mRNA levels were determined by RT TaqMan PCR in UF mononuclear progenitor cells (mean, 0.13 ± 0.07), lin–CD34+CD38+ (mean, 0.21 ± 0.14), and lin–CD34+CD38– (mean, 0.08 ± 0.01) cells. In contrast to the RDR mRNA data, with each stem cell source analyzed, the level of GALV mRNA in lin–CD34+CD38– was lower than in the lin–CD34+CD38+ cells.$

RDR and GALV mRNA levels relative to β2-microglobulin mRNA in human apheresis product, cord blood, and bone marrow. (A) RDR mRNA levels were determined by RT TaqMan PCR in unfractionated (UF) mononuclear progenitor cells (mean, 0.1 ± 0.05), lin^–CD34⁺CD38⁺ (mean, 0.18 ± 0.05), and lin^–CD34⁺CD38^– (mean, 0.20 ± 0.06) cells. (B) GALV mRNA levels were determined by RT TaqMan PCR in UF mononuclear progenitor cells (mean, 0.13 ± 0.07), lin^–CD34⁺CD38⁺ (mean, 0.21 ± 0.14), and lin^–CD34⁺CD38^– (mean, 0.08 ± 0.01) cells. In contrast to the RDR mRNA data, with each stem cell source analyzed, the level of GALV mRNA in lin^–CD34⁺CD38^– was lower than in the lin^–CD34⁺CD38⁺ cells.

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