Flow cytometric analyses of transgene expression in human CD34⁺ PBSCs on day 9 of ex vivo culture. Shown are representative histogram analyses of RD114-MFGS-gp91^phox (A) and RD114-MFGS-CFP (B) transgene expression of transduced (filled histograms) human X-CGD CD34⁺ PBSCs and normal human CD34⁺ PBSCs, in which the gp91^phox and CFP transgene expression is 96% and 97%, respectively. Nontransduced X-CGD CD34⁺ PBSCs served as negative control as represented by the open histogram in each plot.