Figure 3.
![Figure 3. 729HTLVRex- stable cell line contains the Rex mutation and does not produce detectable Rex. (A) HTLV-1 genome fragment containing the Rex start codon was amplified by PCR from genomic DNA of 729wtHTLV-1 and 729HTLVRex- cells. PCR-amplified product was incubated in the presence (+) or absence (-) of SphI, separated on 2% agarose gel, and visualized by ethidium bromide staining. (B) Rex-1 immunoprecipitation. 729, 729wtHTLV-1, and 729HTLVRex- cells were metabolically labeled with [35S] methionine-cysteine. Cell lysates were immunoprecipitated with polyclonal antibody against the HTLV-1 Rex in the presence of protein A-sepharose. The sizes (in daltons, indicated on the left) were determined by comparison to protein markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/12/10.1182_blood-2003-05-1490/6/h82335288003.jpeg?Expires=1735545594&Signature=JyDMPTrE7cFG7pCsTehKjLC~Er7nv3WxI9I3jISo-~1yoj-l~bdRKwF9QFpasSZF~oYrtBn3KRxMO3EdQ2ZVau~~IszpXERuDo64bw4kKD~fmZuREqqJOU5SBbB9e7Y9EyZ2xcHLnp40XH2XYS0G-DVL04Gi1S8diUqdIWtzzsPBogCYZXTR6kiPWYGRCKSMhmsM4slITB3PJHrwy7QBtqm1p~7LXV8CcPH98sK4lRpmmsdwWDC4Na6QW9HGxInGRfY7ziVu8m6YV3m3smU3q49XAKrS3g4~xxm8Egjuz4vFx-gy~-ICqX-2qjoK-FELL6I11kk0T9QInqGMF12oew__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
729HTLVRex- stable cell line contains the Rex mutation and does not produce detectable Rex. (A) HTLV-1 genome fragment containing the Rex start codon was amplified by PCR from genomic DNA of 729wtHTLV-1 and 729HTLVRex- cells. PCR-amplified product was incubated in the presence (+) or absence (-) of SphI, separated on 2% agarose gel, and visualized by ethidium bromide staining. (B) Rex-1 immunoprecipitation. 729, 729wtHTLV-1, and 729HTLVRex- cells were metabolically labeled with [35S] methionine-cysteine. Cell lysates were immunoprecipitated with polyclonal antibody against the HTLV-1 Rex in the presence of protein A-sepharose. The sizes (in daltons, indicated on the left) were determined by comparison to protein markers.
