The effects of signal transduction inhibitors on the transwell migration of Lin^– Sca⁺ Kit⁺ cells. The effects of PI3K inhibitors, LY294002 (100 μM) and wortmannin (500 nM), and Rho kinase inhibitor, Y27632 (50 μM), and toxin B (20 ng/mL) on the migration of Lin^– Sca⁺ Kit⁺ cells were assessed in transwell assays. Cells were preincubated with toxin B for 3 hours and the other inhibitors were included in the top well for the duration of a 6-hour assay. Results are the mean ± SEM of 3 separate experiments and are shown as the percentage inhibition of migration in the absence of inhibitors.