American Society of Hematology

Figure 1.

$Figure 1. WtFLT3 signaling pathways transduce antiapoptotic but not proliferative signals in WtFLT3-32D cells. (A) The growth and viability of WtFLT3-32D cells after IL-3 deprivation. Cells were washed 3 times with RPMI 1640 containing 10% FCS and resuspended with the same medium in the presence or absence of IL-3 (1.0 ng/mL) or FLT3 ligand (FL, 50 ng/mL). Viable cells were counted at the indicated times using the trypan blue dye–exclusion assay. The data shown are means and SDs of 3 independent experiments. (B) DNA histogram of WtFLT3-32D cells (cultured with IL-3, no cytokine, and FL). The percentage of the sub-G1 fraction is indicated.$

WtFLT3 signaling pathways transduce antiapoptotic but not proliferative signals in WtFLT3-32D cells. (A) The growth and viability of WtFLT3-32D cells after IL-3 deprivation. Cells were washed 3 times with RPMI 1640 containing 10% FCS and resuspended with the same medium in the presence or absence of IL-3 (1.0 ng/mL) or FLT3 ligand (FL, 50 ng/mL). Viable cells were counted at the indicated times using the trypan blue dye–exclusion assay. The data shown are means and SDs of 3 independent experiments. (B) DNA histogram of WtFLT3-32D cells (cultured with IL-3, no cytokine, and FL). The percentage of the sub-G₁ fraction is indicated.

This Feature Is Available To Subscribers Only