Survival function of WtFLT3 is mediated by Bad phosphorylation at Ser112 via MEK and Ser136 via Akt. (A) After treatment with 10 μM LY294002 (LY) and 10 μM PD98059 (PD) for 4 hours, each sample was lysed and Western blotting analysis was performed with each antibody. (B) Inhibitory effects of kinase inhibitors on the viability of WtFLT3-32D cells. Treatment with 10 μM LY and 10 μM PD was used. The data shown are means and SDs of 3 independent experiments. (C) Establishment of 3 clones (vector control [▪], wild-type Bad [WtBad; ▧], and double-mutated Bad [DM-Bad; □]) in WtFLT3-32D cells. In the steady state, they were cultured with IL-3 stimulation. (D) IL-3 was washed out and each cell count was carried out at the indicated times after 50 ng/mL FL stimulation. The data shown are the means and SDs of 3 separate experiments.