FLT3 kinase inhibitor induces apoptosis while suppressing Bcl-XL expression and Bad phosphorylation in MOLM-13 cells. (A) Inhibitory effect of AG1296 on the viability of human leukemia cell lines. AG1296 was added at the indicated concentrations, and viable cells were counted by the trypan blue dye–exclusion assay after 48-hour culture. The data shown are means and SDs of 3 independent experiments. (B) In MOLM-13 cells, each cell lysate at the indicated times was immunoblotted by antihuman Bcl-2 and Bcl-XL antibodies. Immunoblotting by antiactin antibody is for the control of total protein level. WL indicates whole lysate. (C) In treatment with AG1296 at increasing doses for 4 hours, each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies.
