![Figure 9. The expression of Bcl-XL and Bad phosphorylation contribute to survival in MOLM-13 cells. (A) In MOLM-13 cells, after 24-hour treatment with Bcl-XL/antisense (Bcl-XL/AS) or Bcl-XL/sense (Bcl-XL/S) oligonucleotides and an additional 4-hour treatment with or without kinase inhibitors (10 μM LY294002 [LY] and 10 μM PD98059 [PD]), each sample was lysed and immunoblotted by anti–Bcl-XL antibody. Each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. Immunoblotting by antiactin antibody is for the control of total protein level. (B) Inhibitory effect of Bcl-XL/AS and kinase inhibitors on the viability of MOLM-13 cells. After 24-hour treatment with each oligonucleotide, LY (10 μM) and PD (10 μM) were or were not applied. The data shown are means and SDs of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/8/10.1182_blood-2002-12-3813/6/h82035076009.jpeg?Expires=1735561598&Signature=cVVFUfVTdeFwtE9PHgGkqJ5PnlgdcaZPOAehfQu7V8qmzLwnYMSKJNj1wmDFjlXp8nJMQSNLzx14IuliNmv4Is25VP8IYW1VQOLwPz2EN-gs7wmdgOnS4R8zFBbpsqLsTrZ61Qn~I~2ikceyrjcZsK~V3PMcBwIBsuSmcwqaFGrtHxRxIlhczb6lshu5Qhn0gCJeuicpsUWPeewMQlD~QclAJfjRb7VdauniBmmiWb3H7Ntv7KO7NpFAe1Z1CDLSKjDUpIpAKBf27uW0vTJbEzZWV6FWGX5-dweIi9eCmD3IAWmTFPgvhCWrgnZEt5HHduRLf5bCeFKPeV7YOOes0A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The expression of Bcl-XL and Bad phosphorylation contribute to survival in MOLM-13 cells. (A) In MOLM-13 cells, after 24-hour treatment with Bcl-XL/antisense (Bcl-XL/AS) or Bcl-XL/sense (Bcl-XL/S) oligonucleotides and an additional 4-hour treatment with or without kinase inhibitors (10 μM LY294002 [LY] and 10 μM PD98059 [PD]), each sample was lysed and immunoblotted by anti–Bcl-XL antibody. Each cell lysate was immunoprecipitated by anti-Bad antibody and immunoblotted by antiphospho Bad specific antibodies. Immunoblotting by antiactin antibody is for the control of total protein level. (B) Inhibitory effect of Bcl-XL/AS and kinase inhibitors on the viability of MOLM-13 cells. After 24-hour treatment with each oligonucleotide, LY (10 μM) and PD (10 μM) were or were not applied. The data shown are means and SDs of 3 independent experiments.
