Figure 1.
Cell adherence renders K562 cells permissive for parvovirus B19 entry and nuclear localization. K562 cells grown in suspension (Susp) could bind parvovirus B19 (A; arrows) in the presence of divalent ions (1 mM Mn2+, 1 mM Mg2+) but did not allow viral entry. Upon spontaneous adherence (Adh), virus entry and nuclear localization were seen in about 7% of K562 cells (B; arrowhead), the extent of which is increased to about 29% upon PMA treatment (C; arrowheads). K562 cells were infected with Cy3-labeled recombinant parvovirus B19 for 30 minutes, fixed, and nuclei stained with SYTO 16 green fluorescent nucleic acid stain. Confocal images were acquired using a Zeiss LSM510 confocal microscope. Original magnification, × 630 for all panels.