IFN-γR2 expression in PHA-activated T lymphoblasts. Surface expression of IFN-γR2 in 5-day PHA-activated T lymphoblasts evaluated by flow cytometry after 48-hour culture in the presence of complete medium or anti–IGF-1R blocking mAb (10 μg/mL) using anti–IFN-γR2 mAb. The histogram represents the expression of IFN-γR2 (gray histogram) or background of mouse IgG1 as negative control (white histogram) in PHA-activated T lymphoblasts. Shown is 1 representative experiment of 3 independently performed. Results in frame are expressed as percentage of positive cells calculated by subtracting the positivity of nonspecific fluorescence detected with isotype-matched control Ig from that obtained with specific fluorescence, and the mean fluorescence intensity (MFI) is indicated.