Figure 2.
Gene expression ofp5n-Iin embryonic RBCs during chicken embryonic development. (A) Northern blot analysis (10 μg RNA per lane); 1 of 3 experiments is shown. (B) The measured chemiluminescence signals of 3 Northern blots (mean ± SD) obtained from 3 different cell pools were compared with the mean enzyme activity of P5N-I (data were taken from Dragon et al16 ). The missing enzyme activity of day-5 RBCs was measured (0.279 ± 0.062 EU/g Hb, n = 3) and recalculated to liter of RBCs with the MCHC of Romanoff. 20 Before day 8, the RNA samples were pooled from the RBCs of several chicken embryos, while the RNA of later days was extracted from the RBCs of single embryos.
