Figure 3.
![Figure 3. The p5n-I expression of day-11 RBCs is induced by cAMP. (A) RBC preparations of day 11 were incubated for 4 and 12 hours with 10 μM NE, compared with a control without incubation (t0). (B) Northern blot analysis of 10 μg RNA per lane hybridized with probes specific for p5n-I and β-globin up to 4 hours with 1 μM NE and 10 μM propranolol (P). (C) RT-PCR result of a representative experiment and the EtBr fluorescence of the RT-PCR products (mean and SD of 3 experiments; **significant difference compared with the control [t0][P < .01]). (D) Result of 4 hours with 10 μM CPCA; RT-PCR result of 1 of 3 experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/12/10.1182_blood-2002-11-3388/6/h82335321003.jpeg?Expires=1735518286&Signature=VYdOO3jQQo-iD-fr5VD8YzG1POsgvNCFc808J4KiXx~XWqZMzWgTiclDDNzaC1VzMBvllDQdn-oP~-jqajX3hCmN34QcfShJbUCuko7xlNSnsJBVYYF32Ywac~n2V6VcAs6gFEthnDvQ~eekkm9ZFud3xhad3yXNtcJLvtHa-gUNKDt7s~hlQWVXGbuTiDS5CzmPZK53Umgz3jcOX8wcsTWI96JrepPwlM82Dz3rYfI11bi4nwhhmlhSj59Wxu7BtPS0z3cmwQjkaIe33v8cUFGIqtfgxcs3-sP5nGGEjtUL~auP42DMSuGXe2Y61M0VqkFNO6vPQCEyQvzqzpZt-Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Thep5n-Iexpression of day-11 RBCs is induced by cAMP. (A) RBC preparations of day 11 were incubated for 4 and 12 hours with 10 μM NE, compared with a control without incubation (t0). (B) Northern blot analysis of 10 μg RNA per lane hybridized with probes specific for p5n-I and β-globin up to 4 hours with 1 μM NE and 10 μM propranolol (P). (C) RT-PCR result of a representative experiment and the EtBr fluorescence of the RT-PCR products (mean and SD of 3 experiments; **significant difference compared with the control [t0][P < .01]). (D) Result of 4 hours with 10 μM CPCA; RT-PCR result of 1 of 3 experiments is shown.
