Microarray analysis of IL-10-inducible genes in macrophages. (A) Genes induced by IL-10 in peritoneal macrophages. Representative results in peritoneal macrophages treated with 10 ng/mL IL-10 for 4 hours are shown in the scatter plot. Similar results were observed in peritoneal macrophages treated with IL-10 for 2 hours, and RAW cells treated for 2 hours and 4 hours. (B) RAW cells and peritoneal macrophages were cultured in the absence or presence of 10 ng/mL IL-10 for the indicated periods. Total RNA was extracted and analyzed by a Northern blot using ³²P-labeled Bcl-3 and SOCS3. Hybridization with a β-actin probe confirmed even loading of RNA in each lane. Data are representative of 3 independent experiments. (C) Peritoneal macrophages isolated from wild-type, IL-10-deficient, and Stat3-mutant mice were treated with 10 ng/mL IL-10 for 2 hours. Total RNA was extracted and analyzed for Bcl-3 and SOCS3 expression by Northern blotting. KO indicates knock-out. (D) Peritoneal macrophages isolated from wild-type mice were treated with 10 ng/mL IL-10 for 2 hours in the presence or absence of 1 μg/mL cycloheximide (CHX) for 0.5 hours. Samples were analyzed by Northern blotting as in panel B.