Nuclear-localized Bcl-3 inhibits the LPS response in macrophages. (A) Schematic structures of the deletion mutants of Bcl-3. ΔN lacks the N-terminal nuclear localization signal. ΔANK lacks the N-terminal 154 residues including the first ankyrin repeat. (B) Lentivirus-infected RAW cells (green) were stained with anti-Bcl-3 (red) and DAPI (blue), and analyzed by confocal microscopy. Scale bar: 2 μm. (C) Lentivirus-infected RAW cells were stimulated with LPS (10 ng/mL) for 6 hours, stained with anti-TNF-α, and analyzed by flow cytometry.