Quantitative real-time RT-PCR to measure changes in gene expression induced by LMP2A. Total RNA was isolated from CD19⁺ B cells from LMP2A transgenic mice and nontransgenic littermates. RNA was reverse transcribed and used in real-time PCR reactions using SYBR green to label double-stranded DNA products. Normalized levels of gene expression in B cells from LMP2A transgenic mice are shown relative to the levels in nontransgenic mice (set to a value of 1). Error bars represent the standard deviations of 4 separate experiments. WT indicates wild type. ^*Transcript levels were compared in splenic B cells, except for PU.1 and PCNA, in which expression was altered only in bone marrow B cells.