American Society of Hematology

Figure 3.

$Figure 3. Novel plasma cell subsets defined by B220 and syndecan-1 expression. (A) E/P-/- cervical lymph node cells from pooled mice were depleted of CD5, Mac-1, and IgM+ cells and the expression of B220 and syndecan-1 (CD138) was determined by flow cytometry. (B) The forward and side light scatter profiles of the 4 major populations present in panel A are displayed as indicated. A representative experiment of 4 is shown. (C) E/P-/- cervical lymph node cells were initially depleted of CD5, Mac-1, and B220 expressing cells (Pre-sep.; ▪). This population was subsequently separated into syndecan-1-positive (Synd1-pos; □) and syndecan-1-lo/negative (Synd1-lo/neg; ▧) fractions as described in “Materials and methods.” The number of cells secreting each of the IgG subclasses (mean ± SD) was determined by ELISPOT for the syndecan-1-separated populations in addition to the unseparated CD5/Mac-1/B220-negative population. A representative experiment of 2 is shown.$

Novel plasma cell subsets defined by B220 and syndecan-1 expression. (A) E/P^-/- cervical lymph node cells from pooled mice were depleted of CD5, Mac-1, and IgM⁺ cells and the expression of B220 and syndecan-1 (CD138) was determined by flow cytometry. (B) The forward and side light scatter profiles of the 4 major populations present in panel A are displayed as indicated. A representative experiment of 4 is shown. (C) E/P^-/- cervical lymph node cells were initially depleted of CD5, Mac-1, and B220 expressing cells (Pre-sep.; ▪). This population was subsequently separated into syndecan-1-positive (Synd1-pos; □) and syndecan-1-lo/negative (Synd1-lo/neg; ▧) fractions as described in “Materials and methods.” The number of cells secreting each of the IgG subclasses (mean ± SD) was determined by ELISPOT for the syndecan-1-separated populations in addition to the unseparated CD5/Mac-1/B220-negative population. A representative experiment of 2 is shown.

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