Increased prominence of peripheral processes in PBT cells transfected with a moesin construct that mimics the phosphorylated form. PBT cells were “nucleofected” with a moesin construct that mimics the unphosphorylated form (moesin-T558A-HA; A-C), or a construct that mimics the phosphorylated form (moesin-T558D-HA; D-F). After 24 hours of culture, the cells were fixed, permeabilized, and stained with Alexa Fluor 568 phalloidin, and anti-HA–FITC. Panels show the confocal images on distribution of actin (B,E; red) and that of the HA-tagged moesin (A,D; green) and corresponding conventional SEM images prepared using chemical drying with tetramethylsilane (C,F). Arrowheads in panels B and E refer to peripheral processes on the cell body representing microvilli. Bar represents 5 μm.