CCCP treatment decreases posttransfusion recovery of functional mouse platelets. (A) Washed platelets were treated for 30 minutes, 60 minutes, or 90 minutes with 100 μM CCCP, labeled with calcein, and infused into mice. Blood was drawn at different time points after infusion and stained for GPIIβ/IIIα. Platelets were identified by PE-fluorescence and forward scatter. Results are shown as percent calcein-labeled platelets ± SEM, n = 5. Similar results were obtained with platelets labeled by biotinylation (not shown). (B, C) Washed platelets were treated with CCCP for the indicated times and platelet responses were tested in standard aggregometry by adding 0.5 U/mL thrombin (B) or 10 μM thromboxane A2 analog U46619 (C). The gray line represents DMSO-treated platelets (90 minutes) activated with the respective agonist. The bar indicates 5 minutes along the x axis. Results are representative of 3 separate experiments.