The effect of 1,25(OH)₂D₃ and 25(OH)D₃ on DC function. On day 1 or day 6 of DC culture, 5 × 10^–8 M 25(OH)D₃ or 5 × 10^–9 M 1,25(OH)₂D₃ (kindly provided by Hoffmann-La Roche, Basel, Switzerland) was added with (right) or without (left) induction of terminal differentiation (A; n = 3). In panel B (n = 2), we compared DCs generated with either 10% FCS (left) or 2% autologous plasma (aut PL; right). After 8 days, antigen presentation was determined by MLR.