Activation of ϵ^ypromoterluciferaseconstructs, CACCCwt orCACCCmut,with or without HS2, by Krüppel-like/SP1-like factors and SCFAs in nonerythroid NIH/3T3 cells. Shown are mean levels of luciferase expression (× 10⁷) from Pwt, HS2Pwt, or HS2Pmut 48 hours after transfection with a combination of transcription factors (EKLF, BKLF, SP1, or SP3), H/FATs, and SCFAs into NIH/3T3 cells. Expression levels between experiments were normalized relative to expression from uninduced HS2Pwt in panel A (0.245 × 10⁷ luciferase units). All points reflect 3 independent transfections with error bars at 1 standard deviation around the mean. (A) Shown is mean luciferase expression from HS2Pwt following cotransfection with EKLF, SP1, SP3, or BKLF plus p300, with (▨) or without (▪) induction by SCFAs. EKLF and SP1 were further tested with PCAF and CBP. Expression under each condition, relative to expression from HS2Pwt in uninduced cells, set at 1, is shown in tabular form beneath the graph, as is fold induction by SCFAs for each paired set of conditions. (B) Shown is mean luciferase expression from uninduced (▪) or induced (▨)Pwt (left) and HS2Pwt (right) when cotransfected with EKLF and p300 or PCAF, as indicated. To preserve detail, scales are different for each data set, as indicated by the slanting line connecting 2 × 10⁷ for each graph. (C) Shown is mean luciferase expression from experiments in which HS2Pmut was cotransfected with EKLF, SP1, SP3, and BKLF plus p300, with () or without (▪) induction by SCFAs. HS2Pwt, in which the CACCC site is intact, was tested in the same experiment with and without EKLF or SCFAs, and is shown at left.