Expression from HS2 when coexpressed with EKLF variants and H/FATs, and SCFAs. Shown are mean levels of expression from HS2Pwt when cotransfected into NIH/3T3 cells with EKLF (EKLFwt) or acetylation-deficient mutants (EKLFmut) EKLF K288R, EKLF K302R, or EKLF K288R/K302R, plus H/FATs and SCFAs, as indicated. Expression was normalized to basal expression from HS2Pwt in Figure 5A (0.245 × 107 luciferase units). Expression from 3 individual transfections per condition was anayzed, and a single standard deviation around the mean is as shown. Basal and SCFA-induced expression from HS2Pwt under each cotransfection condition tested, relative to expression from HS2Pwt alone in uninduced media (set at 1) is shown numerically at “Expression, relative to HS2Pwt.” Fold-induction of expression (SCFA/basal) between paired samples under each condition tested is also shown numerically, at “Fold-induction by SCFAs.” ▪ indicates no induction; and ▨, SCFA induction.
