Gel-shift experiments with ϵ^y-CACCC and β-CACCC sites and SCFA-induced MEL cell nuclear extracts or purified GST-EKLF. (A) Gel-shift experiments in which the radiolabeled 21-bp murine embryonic ϵ^y-CACCCwt DS probe at the left or 22-bp murine adult wt β-CACCC DS probe at the right is incubated with nuclear extracts from SCFA-induced MEL cells. In each subpanel, radiolabeled probes without extracts are seen in the first lane, and incubations with nuclear extracts and 100-fold unlabeled probe, antibodies against BKLF or against EKLF, respectively, are seen in the 2 right lanes of each subpanel. BKLF (band no. 4) and EKLF (band no. 5) DNA-protein complexes are indicated by brackets, and do not produce supershifted complexes. (B) We incubated 10 fmol of 33-bp murine adult β-CACCC, ϵ^y-CACCCwt, or ϵ^y-CACCCmut radiolabeled probe with 20 ng GST-EKLF, and 30-fold (1+) or 50-fold (2+) molar excess of unlabeled probe-specific competitor, as indicated. ^*Indicates that bands were present in gel-shifts with extracts from cells containing the GST backbone only (pGEX-TK, not shown).