Effects of paclitaxel on nuclear translocation of AIF with or without caspase inhibitor IDN-1529. OCI-AML3 cells were treated with 50 ng/mL paclitaxel for up to 48 hours, with or without the caspase inhibitor IDN-1529 (Inh I) (20 μM). Cells were fixed, immunofluorescence stained, and analyzed using a fluorescence microscope and a confocal laser scanning biologic microscope. Images on the left show AIF immunofluorescence staining (FITC-green). Images in the center show nuclei stained with To-Pro 3. Images on the right demonstrate the overlay of AIF and nuclear staining. Green signals surrounding the nuclei represent the cytoplasmic AIF (control and control + IDN-1529 cells). The overlay of green and blue in the nuclei represents the nuclear AIF (Taxol and Taxol+IDN-1529-treated cells). Original magnification is × 90 for all images.