Expression regulation of the putative GR-target genes RIKEN9230106B05, VDUP1, BTG1, and CXCR4 by Epo/SCF and Dex. (A) The fold changes (²log values) of gene expression detected on the hematopoietic microarrays comparing cDNA of factor-depleted I/11 erythroid progenitors treated with Epo/SCF/Dex (ESD) or Epo/SCF/ZK (ESZK) for the indicated times are shown (ESD compared with ESZK). The number of clones (n) representing each of the above genes on the hematopoietic microarray is indicated. (B) Expression of the indicated genes was subsequently determined by real-time RT-PCR. RNA levels of factor-depleted I/11 erythroid progenitors restimulated with ESD or ESZK for indicated times were compared with RNA levels of control cells factor deprived for 4 hours (NF). The fold changes of cytokine/hormone restimulation versus factor deprivation alone are shown as ²log values. (C) RNA levels of proliferating erythroid progenitors derived from either GR^dim/dim fetal livers or wt littermates were compared by real-time RT-PCR. Fold changes of GR^dim/dim over wt are idicated as ²log-values. The –fold regulation is shown as ²log values of fold induction or repression; for instance, a 2-fold induction and 4-fold repression yield values of 1 and –2, respectively. Mean values and standard deviations from 2 separate experiments repeated in duplicate are shown.