Figure 3.
Overexpression of BACH2 enhances cell sensitivity to antileukemic agents. BACH2-overexpressing (no. 67, gray) and control (pDL3, black) RAJI clones were seeded (2 × 105/mL) into 12-well plates and treated with 0.02 μg/mL MTX (A), 0.01 μg/mL VCR (B), 2 μg/mL VP16 (C), 0.2 μg/mL DNR (D), or 0.1 μg/mL Ara-C (E) for 24 hours. After the treatment, cells were stained with PI and analyzed by flow cytometry. The percentage of control (upper bars) and BACH2-overexpressing RAJI cells (lower bars) staining positive with PI (dead cells) after treated with drug was determined. Control cells are indicated by open area, BACH2-overexpressing RAJI cells by filled area. The results are representative of 3 separate experiments.
