Figure 1.
Hypoxia differentially regulates IRP RNA-binding activities. HEK293 cells were grown for the indicated times under hypoxic (1% O2) or normoxic (21% O2, control) conditions. (A) RNA supershift analysis of cytosolic cell extracts using a 32P-labeled Ft IRE and anti-IRP2 antibodies. Experiment was performed 5 times with a representative blot shown. (B) Immunoblot analysis of cytosolic cell extracts with chicken anti-IRP1 antibodies36 or rabbit anti-IRP2 antibodies.37 Df, 100 μM for 16 hours; FAC, 50 μg/mL for 5 hours. The * represents a nonspecific band. IRP1 and IRP2 Western analysis experiments were performed 3 and 4 times, respectively, with representative blots shown.
