Figure 2.
gadd45β is induced rapidly by CD40, and this induction depends on NF-κB. Northern blots showing gadd45β mRNA levels in BJAB cells (A) cocultured at a 1:1 ratio with fibroblasts expressing CD40L or CD70L (Ctrl.) or (B) treated with anti–APO-1. (C) Northern blots showing gadd45β expression in BJAB cells transduced with MIGR1-IκBαM or empty MIGRI and cocultured with CD40L fibroblasts as in (A). Cells were FCM-sorted as in Figure 1C. (D) Northern blots with mRNA extracted from primary mouse splenocytes cocultured at an 8:1 ratio with CD40L-expressing fibroblasts. (E) RT-PCR showing gadd45β induction by sCD40 in B-cell lines. Cells, times, and 32P-labeled probes are indicated.
