Gadd45β does not inhibit killing induced by overexpression of known effectors of Fas apoptotic signaling. HA-Gadd45β and Neo clones were transduced with MIGR1-FADD, MIGR1-CD8:caspase-8, pBabe-tBid, or pBabe-Bak retroviruses or MIGR1 and pBabe controls, and after the times indicated, counted and analyzed for viability by light microscopy and for GFP positivity by FCM. With tBid and Bak, survival rates were extrapolated after gating on cell populations expressing high levels of eGFP. Zero represents the time when retroviral preparations and BJAB cells first came into contact. Values express percentages of viable GFP⁺ cells in cultures expressing proapoptotic molecules relatively to cultures transduced with the corresponding insert-less retrovirus and represent the mean ± standard deviation of 3 independent experiments. Qualitatively similar results were obtained when cell death was scored by FCM using side scatter versus forward scatter plots.