Inhibition of invariant chain expression (CD74) in DCs incubated with antisense oligonucleotides. (A) Day-7 DCs generated from the bone marrow of C57BL/6 mice³⁰  were electroporated with OVA mRNA and 50 mM Ii AS ODNs (AE40) (filled histogram) or control ODNs (SE46) (open histogram).³¹  Cells were replated, cultured for 2 days, and stained with fluorescein isothiocyanate (FITC)-labeled antimouse CD74 (invariant chain) antibody (Ab). Dotted lines indicate DCs stained with FITC-labeled isotype control Ab. To measure total invariant chain expression, cells were permeabilized before Ab staining. (B) Shown is 2-color staining of DCs with phycoerythrin (PE)-labeled anti-CD11c and FITC-labeled anti-CD74, -CD40, -CD80, -CD86, or MHC class II (I-A^b) Ab (PharMingen, San Diego, CA). Data are representative of 5 experiments.