Inhibition of invariant chain synthesis enhances MHC class II presentation of OVA by DCs transfected with OVA mRNA. DCs were transfected with OVA mRNA or a truncated OVA mRNA (tOVA) from which sequences corresponding to the first 40 aa's of the OVA protein were deleted. As indicated, the OVA mRNA-transfected DCs were also treated with Ii AS (AE40) or control (SE40) ODNs. Presentation of the dominant MHC class II (A) and class I (B) OVA epitopes was determined by measuring IL-2 secretion from OVA class II and class I T-hybridomas, respectively.³⁸  Measurements were done in triplicates. Variability is indicated by the error bars. Data are representative of 6 experiments using 2 Ii AS and control ODNs described in “Materials and methods.”