IL-21DCs express low MHC II and CCR7mRNA. (A) DCs, IL-15DCs, and IL-21DCs were analyzed by multicolor flow cytometry (gated on CD11c⁺ cells). Representative data of 10 experiments are shown. The percentage of positive cells is given in each histogram. Unstained cells or isotype control antibodies were used as negative controls. (B) IL-21 enhances CCR1 and CCR5 but suppresses CCR7 expression. Chemokine receptor expression was analyzed in DCs, IL-21-stimulated DCs (100 ng/mL for 24 h), IL-15DCs, or IL-21DCs by PCR.