Figure 3.
Cre-mediated generation of single copy mice from multicopy lines. Genomic DNA was digested with EcoRI and probed with a human 5′Aγ probe and an endogenous mouse CAII probe, as loading control. The sizes of fragments detected are indicated by arrows. The blot includes DNA from all lines used in the single copy expression analysis shown in Figure 4. Original multicopy lines are shown next to the single copy lines obtained after Cre-mediated recombination, except line HPFH-3C where no multicopy DNA sample was available. Single copy β-globin locus lines L72 and L220 were included as copy number controls. LCRAγ single copy lines were generated directly by breeding founder mice with Cre-expressing mice.
