BCR/ABL RNA is expressed in hematopoietic tissues of hCD34tTA-BCR/ABL mice. (A) RNA expression of the oncogene is shown in hematopoietic tissues, including bone marrow, spleen, and thymus, but not from liver or lung detected by Northern blot analysis. RNAfrom the lymph node of a MMTVtTA-BCR/ABL animal that died of pre-B-cell leukemia6 was used as a positive control, whereas bone marrow from a single-transgenic littermate served as a negative control. Total RNA (20 μg) was loaded in each lane and probed with a BCR/ABL-specific probe, followed by hybridization to GAPDH. (B) Result is shown of quantitative real-time PCR to detect and quantify the expression of BCR/ABL in the same animal whose tissue was used for Northern blot analysis in panel A (C814) and from a second double transgenic animal that died of BCR/ABL related disease (C1152).
