Structure of human MEL1 and the 2 gene products. (A) In vitro transcription and translation products of MEL1 and MEL1S. MEL1 and MEL1S cDNAs were transcribed and translated using an in vitro transcription and translation system with rabbit reticulocyte lysates. The products were fractionated on 10% SDS–polyacrylamide gel by electrophoresis. Molecular weights are given in kilodaltons. (B) Comparison of the predicted domain structures between MEL1 and MEL1S. PRD indicates PR domain; DBD1, DNA-binding domain 1; PRR, proline-rich domain; RD, repressor domain; DBD2, DNA-binding domain 2; AD, acidic domain.