Improved fluorescence intensity from SAR-containing vectors. (A) MFI in peripheral blood leukocytes (PBLs) over time after transplantation. Fluorescence from cells transduced with SAR-containing vectors is shown in solid symbols, non–SAR-containing vectors in open symbols. (B) To calculate ratios of MFI in SAR over non–SAR-containing vector transduced cells we used mean fluorescence intensity (MFI) from forward and right-angle light scatter–gated granulocyte (circles) and lymphocyte (triangles) subsets using CELLQuest v3.1 (Becton Dickinson, San Jose, CA) as previously described. 3 A ratio of 1 implies equal fluorescence intensity between SAR versus non-SAR vectors. A ratio of 10 denotes fluorescence 10 times brighter in cells transduced with the SAR-containing vector. Animal designations are noted.
