RBC myosin association with the PVM appears to be G protein-mediated. (A-B) In the absence of AlF, myosin was associated with the RBC membrane and PVM and diffusely distributed in the RBC cytosol. (C-D) AlF perturbs the myosin distribution to an almost exclusive association with the PVM and some structures in the RBC cytosol. (E-F) Immunogold EM revealed myosin to be associated with the PVM and Maurer clefts in AlF-treated IRBC. (G) Western blots showing that AlF causes a loss of myosin from the RBC membrane and stabilization at the PVM. In A-D, 1 mm equals 700 nm. Original magnification E-F, × 20 000.