Repopulation of various hematopoietic lineages and improvement in the T-cell response to influenza following transplantation of mixtures of WT and WASP^— cells in WASP^— mice. (A) The percentages of Ly5.1(WT) and Ly5.2(WASP^—) bone marrow cells infused were as follows: 0% (○), 10% (▵), 20% (♦), 35% (▴), 50% (•) and 100% (▪). Peripheral blood was obtained at the indicated times and analyzed as described in the legend to Figure 1 for T cells, B cells, neutrophils, and monocytes. Shown are the mean and standard errors of data obtained in 2 to 5 animals in each group. Primary challenge with influenza was given at 14 weeks and the secondary challenge was given at 23 weeks. (B) Correction of deficiency of NP-specific T cells by transplantation of WT cells. The mean value for each cohort (▪) is shown, as is the predicted value (•) and standard error calculated by a nonlinear curve-fitting method (see “Materials and methods”) (n = 2-5 animals per cohort; total = 21). (C) Cytokine production by splenic T cells after stimulation with influenza-specific NP tetramer following secondary influenza challenge. Mean (▪) and predicted (•) percentages of cells positive for TNF-α and IFN-γ were calculated as in panel B.