Summary of cytokine production studies of splenic T cells following gene correction. Included in the positive control group were WT mice that did not receive transplants and animals that received transplants of WT cells cocultured with 3T3 cells (M) or cells producing the control MG vector. WASP^— mice and WASP^— mice that received transplants of WASP^— cells transduced with the control MG vector were included in the negative control group. Within each control group and the experimental group, 4 mice were included for the measurements of IL-2 and IFN-γ and 2 for the measurements of TNF-α. The measurements of IL-2 in the CD4 cells for 2 animals were not included in the analysis because of experimental technical difficulties. Spleen cells were stimulated with anti-CD3. For each mouse, assays were performed in duplicate (IL-2, TNF-α)or quadruplicate (IFN-γ). The values shown are the means and standard errors of the average of the measurements in the individual mice. In all cases, cytokine production by the gene-corrected cells was greater than that for the negative controls. When 4 animals were included in the analyses, namely for IFN-γ and IL-2 in CD8 cells and IFN-γ in CD4 cells, the differences between WASP^— and WASP^—/MWG were statistically significant (P < .05).