Cytokine secretion by splenic T cells from gene-corrected WASP^— mice in response to T-cell–receptor stimulation. Splenic T cells from the same animals used in Figure 6B were stimulated with anti-CD3 antibody (500 ng/well) in the presence of Brefeldin A and then analyzed by flow cytometry for IFN-γ and TNF-α accumulation as described in “Materials and methods.” MG is the control vector encoding GFP only and MWG is the bicistronic vector encoding WASP and GFP. (A) The percentage of cells per quadrant is shown. Top right: mouse no. 5 (Figure 6B); bottom right: mouse no. 6 (Figure 6B). (B) Representative studies of IL-2 and IFN-γ expression in the same T-cell preparations. The WASP^—/MWG preparation is from mouse no. 6 (Figure 6B).