Cells lacking Bcr/abl are susceptible to adaphostin-induced apoptosis. (A) ML-1 cells were treated with the indicated concentration of adaphostin or diluent for 8 hours, fixed, stained with PI, and examined for subdiploid cells by flow microfluorimetry. Error bars, ± 1 SD from 4 independent experiments. (B) ML-1 cells were treated with 3 μM adaphostin for the indicated times. Whole-cell lysates were subjected to immunoblotting with C-2-10 antibody, which detects full-length PARP as well as its caspase-generated 89-kDa fragment (arrow head).²⁹  (C) Mononuclear cells isolated from bone marrows of 10 patients with newly diagnosed AML were treated with diluent (-) or 20 μM adaphostin (+) for 48 hours (left panel). Right panel indicates dose-response curve in mononuclear cells from 3 representative AML patients. (D) Lymphocytes isolated from the peripheral blood of patients with CLL were incubated in the presence of diluent or 10 μM adaphostin for 24 hours, stained with PI, and examined for subdiploid DNA content by flow microfluorimetry.