![Figure 6. Transplantation of BM cells in OSMR–/– mice. (A) To investigate whether wild-type hematopoietic cells are able to convert the hematologic profile of mutant mice to the wild type, BM cells were transplanted into busulfan-treated neonatal OSMR–/– mice. To distinguish donor cells from recipients, BM cells from GFP-transgenic mouse were used as input cells. –/– (–/–) indicates OSMR–/– mice reconstituted with BM cells of GFP-transgenic OSMR–/– mouse; –/– (+/+), OSMR–/– mice reconstituted with BM cells of GFP-transgenic OSMR+/+ mouse; and +/+, GFP-transgenic OSMR+/+ mice. Recipient mice were killed 4 months after transplantation and the levels of donor contribution in peripheral blood were determined by FACS. Of fifteen recipient mice, 4 or 5 mice exhibiting relatively high proportion of donor type were used for the following hematologic analyses. (B) Representative FACS profiles of a mouse from each group. (i) –/– (–/–); (ii) –/– (+/+); (iii) +/+; and (iv) non-GFP control. (C-D) Hematologic analyses of the reconstituted OSMR–/– mice and wild-type GFP mice. Orbital plexus blood was collected from anesthetized mice. Peripheral RBCs (C) and platelets (D) were analyzed by using automated counter Sysmex K-4500. The horizontal bar represents the mean. (E-F) GFP-positive donor cells were sorted from BM (E) and spleen (F) of each engrafted recipient mouse. GFP-transgenic wild-type mouse was used as OSMR+/+ control. The hematopoietic progenitor content (GM-CFCs, left panel; and CFU-GEMMs/CFU-MEs/BFU-Es, right panel) of the sorted donor BM and spleen cells was assessed. The figure represents the mean ± SD [n = 4 for –/– (–/–), n = 4 for –/– (+/+) and n = 3 for +/+]. *P < .05 between wild-type and mutant mice. †P < .01 between wild-type and mutant mice.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/9/10.1182_blood-2003-02-0367/6/h82135157006.jpeg?Expires=1735834279&Signature=uiZJhqZU5gm7s9ABXkwxRSI6t0McBIvHtLz0OE4OP87yNKRDJCKWJNNLWZ4QssawD2O4oayn29sRmsfkyL68gTOj9ncJCRDCU0~44uaatf39~e~ieMKSkuwhPcU6u23u5nAyP71GQqrSK8s8eDlM3T8SAolmjJl3RUSK3t~cKpVDj6pIhGIiYUOq1W8HsKJDkSUYxTgniwxvJ-khaSb7w3uXVKbiUtbPt9Up5UiShZKrhqjMLBYgxsa~MGM7Cmig6U-a2R8yD7KyZyUJ13ANZyJPaeBAg5Khg-PSdTIku5uPrrx6dmjjZPjs3fUb-kuCM7~C~J4Ml0CJk87L3C9x3w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transplantation of BM cells in OSMR–/– mice. (A) To investigate whether wild-type hematopoietic cells are able to convert the hematologic profile of mutant mice to the wild type, BM cells were transplanted into busulfan-treated neonatal OSMR–/– mice. To distinguish donor cells from recipients, BM cells from GFP-transgenic mouse were used as input cells. –/– (–/–) indicates OSMR–/– mice reconstituted with BM cells of GFP-transgenic OSMR–/– mouse; –/– (+/+), OSMR–/– mice reconstituted with BM cells of GFP-transgenic OSMR+/+ mouse; and +/+, GFP-transgenic OSMR+/+ mice. Recipient mice were killed 4 months after transplantation and the levels of donor contribution in peripheral blood were determined by FACS. Of fifteen recipient mice, 4 or 5 mice exhibiting relatively high proportion of donor type were used for the following hematologic analyses. (B) Representative FACS profiles of a mouse from each group. (i) –/– (–/–); (ii) –/– (+/+); (iii) +/+; and (iv) non-GFP control. (C-D) Hematologic analyses of the reconstituted OSMR–/– mice and wild-type GFP mice. Orbital plexus blood was collected from anesthetized mice. Peripheral RBCs (C) and platelets (D) were analyzed by using automated counter Sysmex K-4500. The horizontal bar represents the mean. (E-F) GFP-positive donor cells were sorted from BM (E) and spleen (F) of each engrafted recipient mouse. GFP-transgenic wild-type mouse was used as OSMR+/+ control. The hematopoietic progenitor content (GM-CFCs, left panel; and CFU-GEMMs/CFU-MEs/BFU-Es, right panel) of the sorted donor BM and spleen cells was assessed. The figure represents the mean ± SD [n = 4 for –/– (–/–), n = 4 for –/– (+/+) and n = 3 for +/+]. *P < .05 between wild-type and mutant mice. †P < .01 between wild-type and mutant mice.
